Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse 3T3 cells transfected with pCMV-HA-ATCE1-C-del plasmid, driving the expression the nuclear form of ATCE1 vs. controls reveals gene upregulation by the ATCE1 transcription factor

ABSTRACT: ATCE1 is a transcription factor that belongs to the CREB3 subtype of the ATF/CREB transcription factor family. In order to get an indication of the potential promoters ATCE1 targets, a nuclear form was expressed in NIH 3T3 fibroblasts via transfection and upregulated genes were sought after. Experiment Overall Design: NIH 3T3 cells were transfected with either a pCMV-HA-ATCE1-C-del plasmid, driving the expression the nuclear form of ATCE1, or an empty pCMV-HA vector. Experiment Overall Design: The expression level of transcripts isolated from ATCE1 expressing cells was compared to transcripts obtained from cells transfected with an empty vector that was used as a control.

ORGANISM(S): Mus musculus

SUBMITTER: Stelzer Gil

PROVIDER: E-GEOD-2747 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The enigma of ATCE1, an acrosome-associated transcription factor.

Gil Stelzer S Yosef Dicken D Golan Niv N Don Jeremy J

Developmental biology 20060622 1

Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to m ...[more]

PMID: 16925989

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Project description:We have previously shown that adenoviral expression of peroxisome proliferator-activated receptors (PPARs) leads to rapid establishment of transcriptionally active complexes and activation of target gene expression within 5-8 h following transduction. Here we have used the adenoviral delivery system combined with expression array analysis to identify novel putative PPARgamma target genes in murine fibroblasts and to determine the role of the A/B-domain in PPARgamma mediated transactivation of genomic target genes. Of the 257 genes found to be induced by PPARgamma2 expression, only 25 displayed A/B-domain dependency, i.e. significantly reduced induction in the cells expressing the truncated PPARgamma lacking the A/B-domain (PPARgammaCDE). Nine of the 25 A/B-domain dependent genes were involved in lipid storage and in line with this, triglyceride accumulation was considerably decreased in the cells expressing PPARgammaCDE compared to cells expressing full length PPARgamma2. Using chromatin immunoprecipitation (ChIP) we demonstrate that PPARgamma binding to genomic target sites and recruitment of the mediator component TRAP220/MED1/PBP/DRIP205 is not affected by the deletion of the A/B-domain. By contrast, the PPARgamma-mediated CBP and p300 recruitment to A/B-domain dependent target genes is abolished by deletion of the A/B-domain. These results indicate that the A/B-domain of PPARgamma2 is specifically involved in the recruitment or stabilization of CBP and p300 containing co-factor complexes to a subset of target genes. Experiment Overall Design: Total RNA samples were obtained from three individual experiments in which NIH-CAR (i.e. a clonal cell line of NIH-3T3 fibroblasts stably expressing the coxsackie-adenovirus receptor (CARÎ1)) cells were transduced with adenovirus containing empty vector (AdEmpty) followed by addition of vehicle (DMSO) or transduced with adenovirus encoding either full length HA-tagged PPARg2 or A/B-domain deleted HA-PPARgCDE and treated with rosiglitazone.

Dataset Information

Transcription profiling of mouse 3T3 cells transfected with pCMV-HA-ATCE1-C-del plasmid, driving the expression the nuclear form of ATCE1 vs. controls reveals gene upregulation by the ATCE1 transcription factor

Publications

The enigma of ATCE1, an acrosome-associated transcription factor.

Similar Datasets

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