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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Analysis of small RNA population in Arabidopsis drb2 and drb4 mutants

ABSTRACT: We have compared populations of small (21 to 24-nt) RNAs from Arabidopsis immature flowers of WT, drb2 and drb4 mutants and found that DRB2 and DRB4 are needed for the proper biogenesis of polymerase IV-dependent siRNAs Examination of small RNA populations from three backgournd: Col0 (WT), drb2 and drb4 mutants

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jean-Marc Deragon

PROVIDER: E-GEOD-27517 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis.

Pélissier Thierry T Clavel Marion M Chaparro Cristian C Pouch-Pélissier Marie-Noëlle MN Vaucheret Hervé H Deragon Jean-Marc JM

RNA (New York, N.Y.) 20110623 8

Biogenesis of the vast majority of plant siRNAs depends on the activity of the plant-specific RNA polymerase IV (PolIV) enzyme. As part of the RNA-dependent DNA methylation (RdDM) process, PolIV-dependent siRNAs (p4-siRNAs) are loaded onto an ARGONAUTE4-containing complex and guide de novo DNA methyltransferases to target loci. Here we show that the double-stranded RNA binding proteins DRB2 and DRB4 are required for proper accumulation of p4-siRNAs. In flowers, loss of DRB2 results in increased ...[more]

PMID: 21700726

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