Combinatorial binding of transcription factors in the pluripotency control regions of the genome

ABSTRACT: The pluripotency control regions (PluCRs) are defined as genomic regions that are bound by Oct4, Sox2, and Nanog in vivo. We utilized a high-throughput binding assay to record more than 270,000 different DNA/protein binding measurements along incrementally tiled windows of DNA within these PluCRs. This high-resolution binding map is then used to systematically define the context of Oct factor binding and reveals patterns of cooperativity and competition in the pluripotency network. The most prominent pattern is a pervasive binding competition between Oct4 and the forkhead transcription factors. Like many transcription factors, Oct4 is co-expressed with a paralog, Oct1, that shares an apparently identical binding specificity. By analyzing thousands of binding measurements we discover contex

ORGANISM(S): Homo sapiens

SUBMITTER: William Fairbrother 

PROVIDER: E-GEOD-27535 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress