High-resolution analysis of copy number changes in circulating and disseminated tumor cells in breast cancer patients

ABSTRACT: The aim of this study was to establish a single-cell array comparative genomic hybridization (SCaCGH) method providing in-depth genomic analysis of circulating tumor cells (CTCs) and disseminated tumor cells (DTCs). The robustness and resolution limits of the method were estimated with different cell amounts of the breast cancer cell line SKBR3 using 44k and 244k arrays. Subsequent adjustments of the method were conducted analyzing the copy number profiles of 28 CTCs in combination with four hematopoietic cell (HC) controls from eight metastatic patients and of 24 DTCs, three probable HCs, and five HC controls from seven breast cancer patients and one healthy donor. The frequency of the major genomic gains and losses of the analyzed DTC revealed similarities to primary breast tumor samples

ORGANISM(S): Homo sapiens

SUBMITTER: Randi Mathiesen 

PROVIDER: E-GEOD-27574 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress