Querying the genomes of newly isolated un-sequenced Dehalococcoides strains via a microarray targeting the Dehalococcoides genus

ABSTRACT: The genomes of three newly isolated Dehalococcoides strains (11a, 11a5 and MB) were compared against known genomes in the Dehalococcoides genus via a microarray targeting four sequenced Dehalococcoides strains (195, CBDB1, BAV1, and VS). All three strains exhibit different dechlorination patterns, with strains 11a dechlorinating TCE to ethene, 11a5 dechlorinating TCE to VC and MB dechlorinating PCE only to isomers of DCE. Hybridization of their respective genomic DNA to the microarrays showed that the genomes of strains 11a and 11a5 show great similarity to each other and to strains CBDB1 and BAV1 of the Pinellas subgroup, while strain MB shows strong genome similarity to members of the Cornell subgroup. All genes within the respective subgroups that were not detected by microarray are wit

ORGANISM(S): Dehalococcoides

SUBMITTER: Patrick Lee 

PROVIDER: E-GEOD-27611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress