Two phases of mitogenic signaling unveil roles for p53 and EGR1 in elimination of inconsistent growth signals

ABSTRACT: Normal cells require continuous exposure to growth factors, in order to cross a restriction point and commit to cell cycle progression. This can be replaced by two short, appropriately spaced pulses of growth factors, where the first pulse primes a process, which is completed by the second pulse, and enables restriction point crossing. Through integration of comprehensive proteomic and transcriptomic analyses of each pulse, we identified three processes that regulate restriction point crossing: (i) The first pulse induces essential metabolic enzymes and activates p53-dependent restraining processes. (ii) The second pulse eliminates, via the PI3K/AKT pathway, the suppressive action of p53, as well as (iii) sets an ERK-EGR1 threshold mechanism, which digitizes graded external signals into an

ORGANISM(S): Homo sapiens

SUBMITTER: Yotam Drier 

PROVIDER: E-GEOD-27629 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress