Identification and Characterization of Maize MicroRNAs Involved in the Very Early Stage of Seed Germination

ABSTRACT: In this study, a small RNA library from maize seed 24 hours after imbibition was sequenced by the Solexa technology. A total of 11,338,273 reads were obtained. 1,047,447 total reads representing 431 unique sRNAs matched to known maize miRNAs. Further analysis confirmed the authenticity of 115 known miRNAs belonging to 24 miRNA families and the discovery of 167 novel miRNAs in maize. Both the known and the novel miRNAs were confirmed by sequencing of a second small RNA library constructed the same way as the one used in the first sequencing. We also found 10 miRNAs that had not been reported in maize, but had been reported in other plant species. All novel sequences had not been earlier described in other plant species. In addition, seven miRNA* sequences were also obtained. Putative tar

ORGANISM(S): Zea mays

SUBMITTER: Huabang Chen 

PROVIDER: E-GEOD-27664 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress