An integrated genomics approach for novel biomarker discovery in squamous cell cervical carcinoma

ABSTRACT: Detection of copy number dependent gene expression profiles in epithelial tumours has lead to the discovery of genes significant in diagnosis, prognosis and of therapeutic value. We sought to apply the technologies of gene expression profiling and copy number aberration detection by high density oligonucleotide microarray analysis to detect genes with expression profiles regulated by copy number. We surveyed copy number aberrations in 27 squamous cell carcinomas tissues and ten cervical carcinoma cell lines by Agilent 244k aCGH, and in a companion study of 77 tissues we obtained transcriptional profiles of a broader ranger of histologies. Briefly, for normal (NAD) and squamous intraepithelial lesions (SILs), DNA and RNA were extracted from approximately 5 - 25 x 5 mm thin sections of fres

ORGANISM(S): Homo sapiens

SUBMITTER: Ian Roberts 

PROVIDER: E-GEOD-27673 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress