Genome-wide discovery and analysis of microRNAs and other small RNAs from rice embryogenic callus

ABSTRACT: Small RNAs constitute a new and unanticipated layer of gene regulation present in the three domains of life. In plants, all organs are ultimately derived from a few pluripotent stem cells localized in specialized structures called apical meristems. The development of meristems involves a coordinated balance between undifferentiated growth and differentiation, a phenomenon requiring a tight regulation of gene expression. We used in vitro cultured embryogenic calli as a model to investigate the roles of meristem-associated small RNAs. Using high-throughput sequencing, we sequenced 20 million short reads with size of 18-30nt from rice undifferentiated and differentiated calli. We confirmed 50 known microRNA families, representing one third of annotated rice microRNAs. Using a specific computa

ORGANISM(S): Oryza sativa

SUBMITTER: Chong-Jian CHEN 

PROVIDER: E-GEOD-27699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress