EsBAF facilitates pluripotency by conditioning the genome for LIF/STAT3 signaling and by regulating Polycomb function

ABSTRACT: Signaling by the cytokine LIF and its downstream transcription factor, STAT3, prevents differentiation of pluripotent embryonic stem cells (ESCs) by opposing MAP kinase signaling. This contrasts with most cell types where STAT3 signaling induces differentiation. We find that STAT3 binding across the pluripotent genome is dependent upon Brg, the ATPase subunit of a specialized chromatin remodeling complex (esBAF) found in ESCs. Brg is required to establish chromatin accessibility at STAT3 binding targets, in essence preparing these sites to respond to LIF signaling. Moreover, Brg deletion leads to rapid Polycomb (PcG) binding and H3K27me3-mediated silencing of many Brg-activated targets genome-wide, including the target genes of the LIF signaling pathway. Hence, one crucial role of Brg

ORGANISM(S): Mus musculus

SUBMITTER: Raja Jothi 

PROVIDER: E-GEOD-27708 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress