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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Xanthomonas oryzae pv. oryzae (Indian Isolate): Wild type vs. rpfF mutant

ABSTRACT: Transcription profiling of the DSF regulon in Xanthomonas oryzae pv. oryzae (Xoo) using wild type and the rpfF mutant. Cell-cell signaling mediated by the quorum sensing molecule known as Diffusible Signaling factor (DSF) is required for virulence of Xanthomonas group of plant pathogens. DSF in different Xanthomonas and the closely related plant pathogen Xylella fastidiosa regulates diverse traits in a strain specific manner. The transcriptional profiling performed in this study is to elucidate the traits regulated by DSF from the Indian isolate of Xanthomonas oryzae pv. oryzae, which exhibits traits very different from other Xanthomonas group of plant pathogen. In this study, transcription analysis was done between a wild type Xanthomonas oryzae pv. oryzae strain and an isogenic strain that has a mutation in the DSF biosynthetic gene rpfF. Agilent one-color experiment, Organism: Xanthomonas oryzae, Agilent-025096 Genotypic Technology Pvt. Ltd. designed Custom Xanthomonas oryzae 8x15k, Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442).

ORGANISM(S): Xanthomonas oryzae pv. oryzae

SUBMITTER: Genotypic technology

PROVIDER: E-GEOD-27809 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Xanthomonas oryzae pv. oryzae (Indian Isolate): Wild type vs. rpfF mutant

Project description:Transcription profiling of the DSF regulon in Xanthomonas oryzae pv. oryzae (Xoo) using wild type and the rpfF mutant. Cell-cell signaling mediated by the quorum sensing molecule known as Diffusible Signaling factor (DSF) is required for virulence of Xanthomonas group of plant pathogens. DSF in different Xanthomonas and the closely related plant pathogen Xylella fastidiosa regulates diverse traits in a strain specific manner. The transcriptional profiling performed in this study is to elucidate the traits regulated by DSF from the Indian isolate of Xanthomonas oryzae pv. oryzae, which exhibits traits very different from other Xanthomonas group of plant pathogen. In this study, transcription analysis was done between a wild type Xanthomonas oryzae pv. oryzae strain and an isogenic strain that has a mutation in the DSF biosynthetic gene rpfF.

2011-04-02 | GSE27809 | GEO

Xanthomonas oryzae pv. oryzicola (Indian Isolate) wild type and complented strains vs rpfF mutant grown under iron starved condition

Project description:Transcriptional profiling of DSF regulon under iron starvation in Xanthomonas oryzae pv. oryzicola (Xoc; BXOR1) using wild type, rpfF mutant and rpfF mutant with complementing plasmid pSC9. Cell-cell signalling mediated by quorum sensing molecule known as Diffusible signalling factor (DSF) is required for the virulence of Xanthomonas group of plant pathogens. The transcriptional profiling in this study is to elucidate the role of DSF in iron acquisition under the iron limitting environment which would lead to successful colonization and pathogenesis inside host. Agilent one-color experiment, Organism: Xanthamonus oryzicola, (AMADID-041087) Genotypic Technology Pvt. Ltd. designed Custom Xanthamonus oryzicola 8x15k Gene expresssion Array, Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)

2015-01-02 | E-GEOD-53255 | biostudies-arrayexpress

Transcriptomic modulations triggered by Xanthomonas oryzae pv oryzae inoculation in rice

Project description:The transcriptomic modulations leading to defense response in rice one hour after inoculation by Xanthomonas oryzae pv oryzae. Xoo and mock inoculated plant of cultivars IET8585 (bacterial leaf blight resistant) and IR-24 (bacterial leaf blight susceptible) were compared.

2012-03-12 | E-MEXP-3388 | biostudies-arrayexpress

Xanthomonas oryzae pv. oryzicola (Indian Isolate) wild type and complented strains vs rpfF mutant grown under iron starved condition

2015-01-02 | GSE53255 | GEO

Comparative proteome analysis in different media using three Xanthomonas species

Project description:Purpose -Comparison of proteome incubated in different media (rich and minimal media) -Three Xanthomonas species were used (Xanthomonas oryzae pv. oryaze, X. campestris pv. vesicatoria, and X. axonopodis pv. glycines. -Shotgun proteomic was used

2017-10-23 | PXD006310 | Pride

Transcriptome Profiling of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola

Project description:This SuperSeries is composed of the following subset Series: GSE9640: Transcriptome Profiling of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola on two different medias GSE9643: Transcriptome Profiling of Xanthomonas oryzae pv. oryzae knockout mutants at different hybridization conditions and PMTs Keywords: SuperSeries Refer to individual Series

2010-05-26 | E-GEOD-9658 | biostudies-arrayexpress

Comparison of gene expression in rice leaves inoculated with a virulent strain of Xanthomonas oryzae and its mutant deficient in typeIII secretion system.

Project description:Gene expression profiles in rice leaves near the inoculated site of Xanthomonas oryzae and its typeIII secretion mutant were examined. Rice plants (cv Nippnbare, 42 days old) were clip-inoculated with a virulent strain of Xanthomonas oryzae and its △hrcV mutant. At each time points, leaf segments were excised below the clipped site (3 to 5 mm in length) and gene expression profiles were analyzed by microarray.

2015-04-01 | E-GEOD-63047 | biostudies-arrayexpress

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Project description:OsEDS1 is a key regulator of SA-mediated immunity in plants. The OsEDS1 knockout mutant (Oseds1) was characterized and shown to have increased susceptibility to Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), suggesting the positive role of OsEDS1 in regulating rice disease resistance. To identify differentially regulated downstream of Oseds1, we performed transcriptome deep sequencing (RNA-seq) of wild type (ZH11) and Oseds1 inoculated with Xanthomonas oryzae pv. Oryzae (PXO99A).

2023-11-25 | GSE248248 | GEO

Transcription profiling of indica rice cultivar IET8585 (Ajaya) infected with bacterial leaf blight pathogen (Xoo)

Project description:An indica rice cultivar IET8585 (Ajaya), resists diverse races of the Xanthomonas oryzae pv oryzae (Xoo) pathogen attack, and is often cultivated as bacterial leaf blight (blb) resistant check in India. Earlier we reported a recessive blb resistance gene mapped to the long arm of chromosome 5 in IET8585. To further understand the mechanism of recessive and durable resistance response, two indica rice genotypes namely, i) IET8585 (Ajaya), a disease resistant indica veriety from India and ii) IR24, a bacterial leaf blight disease susceptible genotype were selected for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the leaves of the two contrasting rice genotypes under inoculated and un-inoculated conditions during seedling stage. Experiment Overall Design: We used Agilent rice gene chips (G4138A) to investigate the transcript level changes in rice leaf tissues during bacterial pathogen infection. We used two contrasting rice genotypes (IET8585 (Ajaya) blb resistant IR24 blb susceptible) differing in bacterial disease response. Plants were grown growth chambers and inoculated with bacterial pathogen on 18th DAS. Leaf sampling was done in both un-inoculated and inoculated plants at 3 time points. Two replications of microarray experiments were carried out by hybridizing the resistant samples against the susceptible samples.

2008-06-14 | E-GEOD-6244 | biostudies-arrayexpress

Time-resolved combined analysis of transcriptome and proteome of the pathogenicity-activated plant pathogen Xanthomonas oryzae pv. oryzae

Project description:Xanthomonas oryzae pv. oryzae (Xoo) is a rice pathogen causing bacterial blight, which outbreaks in most rice cultivating countries and reduces yield up to 50% due to no effective pesticide. Urgent responses of Xoo upon the initial contacts with rice at infection site are essential for pathogenesis. We studied the time-resolved gene expression of both transcriptome and proteome in the pathogenicity-activated Xoo cells with an in vitro assay system. Genes related to cell mobility, inorganic ion transport and effectors are early response genes to help Xoo cells invade into damaged rice leaf tissues, obtain rare cofactors, and evade rice immune responses. Although the time-resolved gene expression pattern of Xoo is conserved in both mRNA and protein, there are varied time gaps in genes between the expression peaks of mRNA and protein, which implies there is an additional translational selection step of specific mRNAs for rapid translation. The expression pattern of genes from a polycistronic mRNA in the same gene cluster is strictly conserved. The time-resolved gene expression study of Xoo in both transcriptome and proteome provides a valuable information about the pathogenic responses of Xoo at the initial stage of Xoo-rice interaction.

2021-09-09 | PXD020135 | Pride

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