MRNA expression under alkylating condition in wild-type strain and ttha1564 deletion mutant of Thermus thermophilus HB8

ABSTRACT: We analyzed the expression profile of wild-type strain and ttha1564 deletion mutant strain of Thermus thermophils HB8 under alkylating condition. In wild type strain, 107 genes and 96 genes were over 2-fold up- and under 0.5-fold down-regulated by alkylation, respectively. In ttha1564 deletion mutant, 35 genes and 12 genes were over 2-fold up- and under 0.5-fold down-regulated by alkylation, respectively. Keywords: stress response comparison The T. thermophilus HB8 wild-type and delta ttha1564 strains were cultured in TT medium (0.4%(w/v) polypeptone, 0.2%(w/v) yeast extract, 0.1%(w/v) NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, (pH 7.5)) at 70M-BM-0C until the OD600 reached 0.8. The cultures were then divided into two flasks and an equal volume of TT medium prewarmed at 70M-BM-0C was added. O

ORGANISM(S): Thermus thermophilus HB8

SUBMITTER: Hiromasa Ohyama 

PROVIDER: E-GEOD-27818 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress