Extensive Chromatin Remodeling and Establishment of Transcription Factor 'Hotspots' during Early Adipogenesis

ABSTRACT: Adipogenesis is tightly controlled by a complex network of transcription factors acting at different stages of differentiation. Peroxisome proliferator-activated receptor gamma (PPAR gamma) and CCAAT/enhancer binding protein (C/EBP) family members are key regulators of this process. We have employed DNase I hypersensitive site analysis to investigate the genome-wide changes in chromatin structure that accompany the binding of adipogenic transcription factors. These analyses revealed a dramatic and dynamic modulation of the chromatin landscape during the first hours of adipocyte differentiation that coincides with cooperative binding of multiple early transcription factors (including glucocorticoid receptor, retinoid X receptor, Stat5a, C/EBPbeta and -delta) to transcription factor

ORGANISM(S): Mus musculus

SUBMITTER: Susanne Mandrup 

PROVIDER: E-GEOD-27826 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress