The role of the protein kinase-A pathway in the response to alkaline pH stress in yeast

ABSTRACT: Exposure of Saccharomyces cerevisiae to alkaline pH represents a stress condition that generates a compensatory reaction. Here we examine a possible role of the protein kinase-A (PKA) pathway in this response. The phenotypic analysis reveals that mutations that activate the PKA pathway (ira1 ira2, bcy1) tend to cause sensitivity to alkaline pH, whereas its deactivation develops tolerance to this stress. We observe that alkalinization causes a transient decrease in cAMP, the main regulator of the pathway. Alkaline pH causes rapid nuclear localization of the PKA-regulated Msn2 transcription factor which, together with Msn4, mediates a general stress response by binding to STRE sequences in many promoters. Consequently, a synthetic STRE-LacZ reporter shows a rapid induction in response to alk

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Carlos Casado 

PROVIDER: E-GEOD-27925 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress