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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of S. cerevisiae acs2Ts1 mutant

ABSTRACT: Wild-type and the acs2Ts1 mutant yeasts were shifted from 25deg to 37deg. After 60 minutes, Yeasts were harvested and divided into 2 x 2 cell samples. Total RNAs were purified from 4 populations. Experiment Overall Design: microarray data of 2 x 2 = 4 samples were background-corrected with RMA. Because of a global change in expression, the data were taken as 1 x 2 = 2 means and normalized with controls using lowess curve.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hidekazu Takahashi

PROVIDER: E-GEOD-2806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription.

Takahashi Hidekazu H McCaffery J Michael JM Irizarry Rafael A RA Boeke Jef D JD

Molecular cell 20060701 2

Metabolic enzymes rarely regulate informational processes like gene expression. Yeast acetyl-CoA synthetases (Acs1p and 2p) are exceptional, as they are important not only for carbon metabolism but also are shown here to supply the acetyl-CoA for histone acetylation by histone acetyltransferases (HATs). acs2-Ts mutants exhibit global histone deacetylation, transcriptional defects, and synthetic growth defects with HAT mutants at high temperatures. In glycerol with ethanol, Acs1p is an alternate ...[more]

PMID: 16857587

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