Affymetrix SNP 6.0 array data for single cell multiple displacement amplification

ABSTRACT: Generating sufficient DNA for high-throughput genetic analysis has always been a challenge for clinical settings where the amount of source DNA is limited. Multiple displacement amplification (MDA) has been proposed as a promising candidate for such situations. Previous work with lower-resolution arrays confirmed the utility of single-cell MDA products for large-size (~30 Mb) genome variation screening. We tested the performance of single-cell MDA products on the SNP 6.0 arrays to examine the performance of single-cell MDA in SNP genotyping, copy number polymorphism, de novo copy number variation (CNV) and loss of heterozygosity (LOH) analysis. Our data show that for SNP genotyping, single-cell MDA did not obtain complete genome coverage or high sequence fidelity. For CNV calling, single-c

ORGANISM(S): Homo sapiens

SUBMITTER: Yan Shea (Xie) 

PROVIDER: E-GEOD-28132 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress