Diet study with EE2 in liver of female largemouth bass (LMB)

ABSTRACT: 17alpha-ethinylestradiol (EE2) is one of the most potent estrogens that have the ability to interfere with the endocrine system of fish. The objective was to investigate the effects and mechanisms of action caused by 60 days of dietary exposure to 0.2 mg EE2/kg and 0.07 mg EE2/kg feed in female largemouth bass (LMB) during the reproductive season. Microarrays and pathway analyses were performed on hepatic tissues to identify genes and biological processes altered in female LMB by EE2 exposure. The hypothesis was that the two concentrations of EE2 would produce dose-response changes in sensitive genes. Body and ovary weights were measured and blood was collected for measurement of plasma steroid hormones (17beta-estradiol (E2), testosterone (T)) and vitellogenin (VTG) using ELISA. The 0.2 m

ORGANISM(S): Micropterus salmoides

SUBMITTER: Reyna Colli-Dula 

PROVIDER: E-GEOD-28143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress