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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from Medicago truncatula roots treated with S. meliloti wild-type or exoA mutant bacteria, or auxin transport inhibitors

ABSTRACT: Publication title: Pseudonodule formation by wild type and symbiotic mutant Medicago truncatula in response to auxin transport inhibitors This SuperSeries is composed of the following subset Series: GSE27991: Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors GSE28171: Expression data of Medicago truncatula Jemalong A17 roots treated with S. meliloti exoA mutant or auxin transport inhibitors GSE28172: Expression data of Medicago truncatula skl1-1 roots treated with S. meliloti wild-type or auxin transport inhibitors GSE28173: Genes differentially expressed in wild-type Medicago truncatula plants during nodulation Refer to individual Series

ORGANISM(S): Medicago truncatula

SUBMITTER: Adriana Rightmyer

PROVIDER: E-GEOD-28174 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nitrogen fixation mutants of Medicago truncatula fail to support plant and bacterial symbiotic gene expression.

Starker Colby G CG Parra-Colmenares Adriana L AL Smith Lucinda L Mitra Raka M RM Long Sharon R SR

Plant physiology 20060111 2

The Rhizobium-legume symbiosis culminates in the exchange of nutrients in the root nodule. Bacteria within the nodule reduce molecular nitrogen for plant use and plants provide bacteria with carbon-containing compounds. Following the initial signaling events that lead to plant infection, little is known about the plant requirements for establishment and maintenance of the symbiosis. We screened 44,000 M2 plants from fast neutron-irradiated Medicago truncatula seeds and isolated eight independent ...[more]

PMID: 16407449

Publication: 1/2

Similar Datasets

Expression data of Medicago truncatula Jemalong A17 roots treated with S. meliloti exoA mutant or auxin transport inhibitors

Project description:Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti. Three to five independent biological replicates were performed for each treatment (buffer, exoA bacteria and ATI) at 21 days after treatment.

2011-03-25 | E-GEOD-28171 | biostudies-arrayexpress

Genes differentially expressed in wild-type Medicago truncatula plants during nodulation

Project description:We used an Affymetrix oligonucleotide microarray consisting of 9,935 tentative consensus (TC)sequences, which are based on cDNA libraries (Mitra et al., 2004 [PMID:15220482]). We examined gene expression of wild-type M. truncatula plants after inoculation with wild-type S. meliloti at 1 d, 4 d, 7d, 14 d, and 21 d. We chose these time points because they span the range of development of the Rhizobium-legume symbiosis, from initiation of the interaction through nitrogen fixation. The gene expression data for the 1-day time point (Mitra et al., 2004 [PMID 15220482]) and the other time points was previously published (Starker el al., 2006 [PMID 16407449]). Three or five independent biological replicates were performed at each time point (1, 4, 7, 14 and 21 days after treatment) and each treatment (buffer and wild-type bacteria).

2011-03-26 | E-GEOD-28173 | biostudies-arrayexpress

Expression data of Medicago truncatula Jemalong A17 roots treated with auxin transport inhibitors

Project description:Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti. Three independent biological replicates were performed at each time point (1 and 7 days after treatment) for each treatment (buffer and ATI).

2011-03-26 | E-GEOD-27991 | biostudies-arrayexpress

Expression data of Medicago truncatula skl1-1 roots treated with S. meliloti wild-type or auxin transport inhibitors

Project description:Rhizobium and allied bacteria form symbiotic nitrogen-fixing nodules on legume roots. Plant hormones appear to play a role in nodule formation. We treated Medicago truncatula roots with auxin transport inhibitors (ATIs) N-(1-naphthyl)phthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) to induce the formation of pseudonodules. We compared the transcriptional responses of M. truncatula roots treated with ATIs to roots inoculated with Sinorhizobium meliloti. The transcriptional response of M. truncatula roots 1 and 7 days after ATI treatment were opposite to roots treated with S. meliloti. Three independent biological replicates were performed for each treatment (buffer, ATI and wild-type bacteria) at 21 days after treatment.

2011-03-25 | E-GEOD-28172 | biostudies-arrayexpress

Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress

Project description:we used two-dimensional gel electrophoresis and mass spectrometry to characterize the proteome-level changes associated with salt stress response in Medicago sativa cv. Zhongmu-1 and Medicago truncatula cv. Jemalong A17 roots. The tandem mass spectrometry analysis of the differentially accumulated proteins resulted in the identification of 60 and 26 proteins in Zhongmu-1 and Jemalong A17 roots, respectively.

2016-04-14 | PXD003761 | Pride

Transcription profiling of Medicago trunculata leaf tissue over-expressing Legume Anthocyanin Production

Project description:Transcription profiling of leaf tissue from Medicago truncatula over-expressing Legume Anthocyanin Production.

2009-10-13 | E-MEXP-1854 | biostudies-arrayexpress

Medicago truncatula roots. Nodulation vs. Arsenic

Project description:Legumes, in interaction with resistant rhizobia, combined both moderate tolerance and accumulation of metal(loids) in roots, with the ability to grow without nitrogen supply (Pajuelo et al., 2011). This quality has attracted attention for phytostabilisation of polluted soils (Reichman, 2007). Physiological studies suggest that low arsenite concentrations lead to a decrease of nodulation process (Dary et al., 2010; Pajuelo et al., 2008). Moreover, Lafuente et al. (2010) described a reduction in the expression patterns of nodulins genes in the presence of arsenite. Nevertheless, a global transcriptomic analysis has never been approached. In order to decipher the genetic regulation underlying the arsenite effect on the model symbiotic interaction Medicago-Sinorhizobium, we have performed a meta-analysis of three different hybridizations. These compare transcriptomic profiles of roots cultivated under different treatments (M-125 M-5M arsenite, M-1rhizobia).

2014-12-08 | E-MTAB-1723 | biostudies-arrayexpress

Transcription profiling of transgenic seeds of Medicago truncatula after introduction of mannan synthase transgene from guar

Project description:Gene expression profiling of transgenic seeds of Medicago truncatula after introduction of mannan synthase transgene from guar.

2008-08-28 | E-MEXP-1316 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula TTG1 over-expressing hairy root

Project description:Transcriptomics analysis of MtTTG1 over-expressing hairy root in the background of Medicago truncatula genotype Jemalong A17.

2009-08-14 | E-MEXP-1758 | biostudies-arrayexpress

High-throughput sequencing of small RNAs in Medicago truncatula

Project description:Medicago truncatula endogenous small RNAs The dataset contains Medicago truncatula Gaertn. cv. Jemalong endogenous small RNA sequences in the range 18-28 nucleotides. High-throughput Solexa/Illumina sequencing was carried out at the Sainsbury Laboratory, Norwich, UK. Please see www.illumina.com for details of the technology. Small RNA sequences were mapped to Medicago truncatula genome release 2.0 (http://www.medicago.org/genome/), the number of matches to the unfinished genome, if any, is recorded in the Series supplementary file GSE13761_sequence_annotations.txt.gz. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2009-11-09 | E-GEOD-13761 | biostudies-arrayexpress

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