Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from transgenic rice with OsEATB

ABSTRACT: Rice ethylene-response AP2/ERF factor OsEATB restricts internode elongation by down-regulating gibberellin biosynthetic gene Leaf tissues of 4-leaf-old transgenic and nontransgenic seedlings (10 plants each), respectively, were selected.

ORGANISM(S): Oryza sativa

SUBMITTER: weiwei qi

PROVIDER: E-GEOD-28229 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression data from LRK1 060615 transgenic rice and control 9311

Project description:Plant height is a critical constituent of plant architecture. Rice (Oryza sativa) plants have the potential to undergo rapid internodal elongation, which determines plant height. A number of physiological studies have proved that gibberellin is involved in internode elongation. Leucine-rich repeat receptor-like kinases (LRR-RLKs) are the largest subfamily of transmembrane receptor-like kinases in plants. Plant LRR-RLKs play important functions in mediating a variety of cellular processes and regulating responses to environmental signals. LRK1, a PSK receptor homolog, is a member of the LRR-RLK family. In the present study, differences in ectopic expression of LRK1 were consistent with extent of rice internode elongation. Analyses of gene expression demonstrated that LRK1 restricts gibberellin responsiveness during the internode elongation process by down-regulation of the gibberellin biosynthetic gene, ent-KAURENE OXIDASE (OsKO2). Leaf tissues of 6-week-old LRK1 060615 transgenic rice and control 9311 rice (10 plants each) were selected.

2012-02-12 | E-GEOD-35729 | biostudies-arrayexpress

Expression data from transgenic rice with anti-OsPDCD5

Project description:Environmental stresses influence the growth of plants and the productivity of crops. Salinity is one of the most important abiotic stresses for agricultural crops. PCD is induced by various biotic and abiotic stresses in algae and higher plants, including high salinity treatment. OsPDCD5, an ortholog to mammalian-programmed cell death 5, is up-regulated under low temperature and NaCl treatments. We found that the transgenic rice which constitutively expressed anti-OsPDCD5 increased salt stress tolerance in unique ways. By using the Rice Genome Microarray, we identi?ed target genes that were regulated in transgenic rice plants by anti-OsPDCD5. Leaf tissues of 2-week-old transgenic and nontransgenic seedlings (10 plants each) before 200mM NaCl treatment, 20mins and 3 hours after 200mM NaCl treatment, respectively, were selected.

2011-11-01 | E-GEOD-28209 | biostudies-arrayexpress

Expression data from OsEBS-transgenic rice B10201 and B10301 and control Guichao2

Project description:In rice (Oryza sativa L.), the number of panicles, spikelets per panicle and grain weight are important components of grain yield. These characteristics are controlled by quantitative trait loci (QTLs) and are derived from variation inherent in crops.The identification of different yield related QTLs facilitates an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. In the present study, We cloned and characterized a large-panicle QTL, and confirmed that the newly identified gene OsEBS (enhancing biomass and spikelet number) increased plant height, leaf size and spikelet number per panicle, leading to an average of 37.62% increase in total grain yield per plant. trait loci (QTLs) and are derived from variation inherent in crops. OsEBS-transgenic rice B10201 and B10301 and control Guichao2

2013-05-04 | E-GEOD-46616 | biostudies-arrayexpress

Study the function of miR-195 and miR-30d in prostate cancer cell (LNCap and DU145)

Project description:miRNAs are related with the initiation and development of prostate cancer. We discover the miR-195 and miR-30 can be as a biomarker of prognosis of prostate cancer in clinical patients. miRNA functions through affecting the mRNA degradation by binding the mRNA 3âUTR. So we test the change of transcriptional profile of miR-195 and miR-30d cell line respectively to further study the function of miR-195 and miR-30d. To study the function of miR-195 and miR-30d in prostate cancer, we setup the over-expression cell line of the miR-195 and miR-30d respectively in prostate cancer cell(LNCap and DU145), then study the change of transcriptional profile in cell line by microarray experiment (Affymetrix PrimeView human gene expression). We order the over-expression plasmid of vector, miR-195 and miR-30d from System Biosciences company (Cat No: Scramble Vector PMIRH000PA-1 as Control, miR-195 PMIRH195PA-1, miR-30d PMIRH30dPA-1), and packaged the virus and construct the stable cell line (LNCaP_Control, LNCaP_mir195, LNCaP_mir30d,DU145_Control, DU145_mir195, DU145_mir30d,). We test the transcriptional profile in cell line by microarray experiment (Affymetrix PrimeView human gene expression).

2016-08-23 | E-GEOD-85884 | biostudies-arrayexpress

To detect change of gene expression after knock-down of hormone receptor in hepatocellular carcinoma cell line

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-01-19 | E-GEOD-65081 | biostudies-arrayexpress

Hsp27 acts as a master molecular chaperones and plays an essential role in hepatocellular carcinoma metastasis

Project description:Hsp27 can regulate multiply signaling pathway and protect HCC cells apoptosis by mediating interaction with its cochaperones 3 HepG2 samples stably transfected with Hsp27 ORF vectors, Hsp27 shRNA or control vectors

2012-05-21 | E-GEOD-38080 | biostudies-arrayexpress

Retinal whole genome microarray analysis after irradiated injury of intraorbital nerve in the monkey

Project description:To obtain and analyze early retinal changes at molecular level at 24h after ipsilateral intraorbital nerve radiation injury by gamma knife surgery Unilateral intraorbital optic nerves of three Rhesus Macaques were treated by gamma knife surgery (GKS) with irradiated doses of 15Gy, while the contralateral optic nerves and retinas served as the control. Gene expression profiles of control and affected retinas were analyzed with Affymetrix Rhesus Macaque Genome arrays at 24h after treatment

2011-09-29 | E-GEOD-29901 | biostudies-arrayexpress

To detect change of gene expression after knock-down of hormone receptor in hepatocellular carcinoma cell line

Project description:Vector or shRNA was transfected into SMMC-7721 to detect effect of shRNA on gene expression Specific hormone receptor was knocked down by shRNA in hepatocellular carcinoma

2015-01-13 | E-GEOD-64890 | biostudies-arrayexpress

compare the difference of gene expression between wild type ESCs and Mll2 Knockdown ESCs

Project description:Mll2 (ALR) is a histone 3 lysine 4 trimethyltransferase to function as gene activation.In our study, we found that Mll2 is vital for proper control of proliferation and lineage differentiation of mouse ESCs, particularly towards the cardiac-specific lineages. We used microarrays to detail the global programme of gene expression to compare the difference after Mll2 knockdown in E14 cell lines. E14 cells were infected with lentiviruses expressing short hairpin RNA (shRNA) for Mll2 for 24 hours, and then puromycin was added at 2mg/ml to select for 5 days. Then collet samples of control and Mll2 knockdown E14 cells for RNA extraction and hybridization on Affymetrix microarrays.

2014-01-25 | E-GEOD-54382 | biostudies-arrayexpress

Gene expression regulated by HBV mRNAs

Project description:HBV mRNAs harboring miR-122 response elements were found to bind and sequester endogenous miR-122. This microarray experiment was carried out to find out differentially expressed genes in Huh7 cells expressing HBV mRNAs. RNA was extracted from Huh7 cells transfected with pcDNA3.1 plasmid containing HBV mRNA with wild type (WT) or mutant (Mut) miR-122 response elements in its 3M-bM-^@M-^Y-UTR or the empty pcDNA3.1 vector (3.1) as a mock. Total RNA was then extracted and processed on Affymetrix microarrays in biological duplicate.

2014-12-12 | E-GEOD-64084 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data