Tripartite organization of centromeric chromatin in budding yeast

ABSTRACT: The centromere is the genetic locus that organizes the proteinaceous kinetochore and is responsible for attachment of the chromosome to the spindle at mitosis and meiosis. In most eukaryotes, the centromere consists of highly repetitive DNA sequences that are occupied by nucleosomes containing the CenH3 histone variant, whereas in budding yeast, an ~120-bp Centromere DNA Element (CDE) that is sufficient for centromere function is occupied by a single right-handed CenH3 (Cse4) nucleosome. However, these in vivo observations are inconsistent with in vitro evidence for left-handed octameric CenH3 nucleosomes. To help resolve these inconsistencies, we characterized yeast centromeric chromatin at single base-pair resolution. Intact particles containing both Cse4 and H2A are precisely protected

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jorja Henikoff 

PROVIDER: E-GEOD-28298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress