Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

12 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Zebrafish 22 hpf embryos: WT vs. MO-grnA injected

ABSTRACT: Transcriptional profiling of zebrafish embryos comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for the determination of expression profiling at 22 hpf under GRN-A deficiency. Two-condition experiment: wild type vs. MO-grnA treated cells. 3 biological replicates: each group contains 200 embryos.

ORGANISM(S): Danio rerio

SUBMITTER: gen lin

PROVIDER: E-GEOD-28318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos

Project description:Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for determination of expression profiling of trunk muscle at 16, 24, 48, 72 hpf under GRN-A deficiency. Two-condition experiment, wild type vs. MO-grnA treated cells. Biological replicates: each group contains 200 embryos.

2013-06-01 | E-GEOD-38441 | biostudies-arrayexpress

Zebrafish 22 hpf embryos: WT vs. MO-grnA injected

Project description:Transcriptional profiling of zebrafish embryos comparing wild type untreated embryos with embryos injected with morpholino of zf-grna. This assay is used for the determination of expression profiling at 22 hpf under GRN-A deficiency.

2013-03-28 | GSE28318 | GEO

WT vs MO-grnA injected: 16, 24, 48, 72 hpf trunk region of zebrafish embryos

2013-06-01 | GSE38441 | GEO

The functional study of zebrafish Bad gene

Project description:Transcriptional profiling of zebrafish embryo comparing wild type untreated embryos with embryos injected with morpholino of zf-bad. This assay is used for determination of expression profiling at 24 hpf and 48 hpf under Bad deficiency. Two-condition experiment, wild type vs. MO-Bad treated cells. Biological replicates: each group contains 200 embryos.

2014-06-01 | E-GEOD-47971 | biostudies-arrayexpress

The functional study of zebrafish rig1 gene in NNV infection

Project description:Transcriptional profiling of ZF-4 cells comparing controle morpholino-infection cells with rig1 morpholino-infection cells. This assay is used for determination of gene expression profiling at 6 hpi under NNV infection in rig1 knockdown cells. Two-condition experiment, controle morpholino-NNV infection cells vs. rig1 morpholino-NNV infection cells (5 MOI).

2015-05-12 | E-GEOD-57599 | biostudies-arrayexpress

Zebrafish embryos: hdac1 Morphants vs Standard control morphants at 12, 18 and 27 hpf

Project description:Transcriptional profiling of hdac1 ATG morphant zebrafish embryos in comparison to standard control injected embryos. Embryos where injected at the one cell stage with either a translation blocking morpholino (Hdac1 ATG) or Standard control morpholino (StCo). RNA was extracted at 12, 18 and 27hpf from both sets of embryos and compared with a two-colour hybridisation. Timecourse experiment, hdac1 ATG morphants vs. standard control morphants. Biological replicates: 3

2011-01-21 | E-GEOD-26709 | biostudies-arrayexpress

RNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs

Project description:RNASeq of Arg2 gRNA or scrambled gRNA CRISPR'd Tregs

| PRJNA588065 | ENA

Cas9 gRNA engineering for selectable genome editing, activation and repression

Project description:RNA-Seq after Cas9-gRNA transfection with different length gRNAs we performed PolyA Selection and RNA-Seq on cells transfected with dCas9-VPR and a gRNA of each length (20nt, 16nt, or 14nt) targeting ACTC1, MIAT, or HBG1/2

2015-07-15 | E-GEOD-70694 | biostudies-arrayexpress

Progranulin A mediated MET signalling is essential for liver morphogenesis in zebrafish

Project description:Regulation of embryonic liver growth remains largely elusive. Progranulin has been discussed in pathological liver growth; however, the functional role of Pgrn in embryonic liver growth has never been addressed. Knockdown of GrnA, the orthologue of mammalian pgrn in zebrafish, displayed a deficient hepatic outgrowth during hepatogenesis. Expression profiles manifested that pgrn-deficiency impaired hepatogenesis associated with dysregulation of Met signaling. Pgrn regulates hepatic expression of Met was further verified in vitro and in vivo. These results indicate that Pgrn is a novel factor required for embryonic hepatic outgrowth and reveal a novel link between Pgrn and Met signaling. To explore the GrnA induced genomic responses during hepatic outgrowth, mRNA expression profiles were compared from grnA morphants and control embryos using zebrafish 14K oligonucleotide microarray at 72 hpf, when hepatocytes were rapid proliferating.

2010-11-10 | E-GEOD-19211 | biostudies-arrayexpress

Gene expression profile in NNV infected ZF-4 cells

Project description:Transcriptional profiling of ZF-4 cells comparing mock infection cells with cells infected with NNV. This assay is used for determination of gene expression profiling at 6 hpi under NNV infection. Two-condition experiment, Mock infection vs. NNV infection (5 MOI).

2015-05-12 | E-GEOD-57600 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data