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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Miz-1 is required to coordinate the pre-TCR-dependent transitional step in DN3/DN4 thymocytes

ABSTRACT: Lineage negative, CD44 negative, CD25 positive thymocytes were isolated from wt mice or Miz1 POZ-domain knockout mice to analyze the effect of loss of Miz1 in the DN3 population of T-cells We used the mouse Affymetrix MOE430-2 microarray to characterize global gene expression changes of DN3 thymocytes from Miz1 knockout mice. We performed microarray based gene expression profiling to determine the effect of loss of Miz1 activity on the gene expression pattern of mouse DN3 thymocytes from wt and Miz1-delta-POZ mice.

ORGANISM(S): Mus musculus

SUBMITTER: Lothar Vassen

PROVIDER: E-GEOD-28342 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Miz-1 is required to coordinate the pre-TCR-dependent transitional step in DN3/DN4 thymocytes

Project description:Lineage negative, CD44 negative, CD25 positive thymocytes were isolated from wt mice or Miz1 POZ-domain knockout mice to analyze the effect of loss of Miz1 in the DN3 population of T-cells We used the mouse Affymetrix MOE430-2 microarray to characterize global gene expression changes of DN3 thymocytes from Miz1 knockout mice.

2011-08-24 | GSE28342 | GEO

Expression data from WT DN3, TCF-1-deficient DN3 thymocytes, and T cell lymphomas in TCF-1-deficient animals.

Project description:TCF-1 is an HMG family transcription factor which is known to be critical for T cell development. We discovered that it has a unique role in suppressing malignant transformation of developing thymocytes at early stages. We identified ID2 and LEF-1 as key TCF-1 target genens in tumor suppression. We used microarrays to detect gene expression changes in WT and TCF-1 deficient DN3 thymocytes as well as T cell lymphoma cells developed in TCF-1 KO mice. DN3 thymocytes were directly sorted from WT or TCF-1 KO mice. T cell lymphoma blast cells were also sorted from TCF-1 KO mice that developed the disease. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).

2012-10-25 | E-GEOD-33292 | biostudies-arrayexpress

Miz1 is required to maintain physiological levels of autophagic flux_1

Project description:Mice: knock-out of the Miz1 POZ-domain in brain (Miz1DPOZNes) and control mice. The RNA expression in cerebella of of old Miz1 delta POZ mice (1.5 years), old control mice (1.5 years), young Miz1 delta POZ mice (5.5 weeks) and young control mice (5.5 weeks) was compared

2013-10-01 | E-MEXP-3878 | biostudies-arrayexpress

Identification of the molecular targets of miR-185 in DN3 stage thymocytes

Project description:T cell-specific overexpression of miR-185 caused a developmental block at DN3 stage of thymopoiesis. DN3 stage thymocytes were sorted from the wild type (C57BL/6) and miR-185 Tg mice, followed by total RNA isolation.

2013-10-28 | E-GEOD-49057 | biostudies-arrayexpress

Genome-wide maps of chromatin accessibility in DN3 thymocytes and splenic naïve CD4 T cells.

Project description:We found that naïve T cells from SCD1-/- mice have an intrinsic preference for regulatory T cells differentiation. Such preference was imprinted to DN3 thymocytes developed in SCD1 deficient thymus and persisted to mature T cells. To investigate the potential epigenetic changes underlying the Treg-bias. We employed ATAC-seq to examine the chromatin accessibility of naïve T cells and DN3 thymocytes from SCD1-/- mice and their littermate controls.

2023-09-23 | GSE209563 | GEO

Role of Tcf-1 (Tcf7) in DN3 thymocytes

Project description:Comparison of wild-type and Tcf-1-deficient DN3 thymocytes

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Role of GATA in DN3 stage thymocytes

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Elevated expression of the H3K36me2 demethylase Kdm8/Jmjd5, a direct Miz1 target gene causes prolonged postnatal Schwann cell proliferation in the murine Miz1∆POZ neuropathy

Project description:The deletion of the POZ domain of the transcription factor Miz1 leads to a late onset neuropathy with subsequent spontaneous regeneration in mice. In this study, we aim to identify genes that are involved in the development of this neuropathy. We traced first gene regulatory changes in sciatic nerve tissue from Miz1∆POZ mice to 30 days after birth. At this time point, we harvested samples of sciatic nerve tissue from several mice and performed RNAseq analysis to identify differentially expressed genes between Miz1∆POZ and wild type mice.

2018-03-14 | E-MTAB-5600 | biostudies-arrayexpress

Expression data from WT DN3, TCF-1-deficient DN3 thymocytes, and T cell lymphomas in TCF-1-deficient animals.

2012-10-25 | GSE33292 | GEO

Cytoplasmic Miz1 Suppresses TNF-α-Induced NF-κB Activation and Hepatocellular Carcinoma Progression via the Interaction with Metadherin

Project description:Previous studies have revealed that nuclear Miz1 is implicated in the regulation of several cancers mainly relying on its POZ domain. Current study, however, uncovers an unexpected function of cytoplasmic Miz1 in hepatocellular carcinoma (HCC). In vivo HCC models in Miz1 and Miz1(POZ) KO mice along with RNA-sequencing experiments show that hepatocyte specific ablation of Miz1 exacerbates TNF-α-induced NF-κB activation and promotes the progression of HCC independent of its transcriptional activity. Ubiquitination and proteosomal degradation of cytoplasmic Miz1 liberates its novel partner Metadherin (MTDH), and its site specific phosphorylation is critical to NF-κB activation. These findings reveal a brand new role of cytosolic Miz1 as a suppressor of HCC, and provide profound insights into liver cancer diagnosis and treatment.

2020-10-11 | GSE104940 | GEO

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