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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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SMAD3 ChIP-on-chip on A549 cell line

ABSTRACT: The aim of this study is to identify SMAD3 binding targets affected by the TGFb1/SMAD3 signal transduction on a genome-wide scale Formaldehyde cross-linked, sonicated chromatin was prepared from non-stimulated A549 cell line and stimulated with TGFb1. Chromatin immunoprecipated with anti-SMAD3 antibody is labeled with Cy5 and mock IP (with anti-FLAG antibody) is labeled with Cy3 and co-hybridized on Agilent Human Promoter Set arrays. Two biological replicates were performed.

ORGANISM(S): Homo sapiens

SUBMITTER: Kusum Pandit

PROVIDER: E-GEOD-28346 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High throughput determination of TGFβ1/SMAD3 targets in A549 lung epithelial cells.

Zhang Yingze Y Handley Daniel D Kaplan Tommy T Yu Haiying H Bais Abha S AS Richards Thomas T Pandit Kusum V KV Zeng Qilu Q Benos Panayiotis V PV Friedman Nir N Eickelberg Oliver O Kaminski Naftali N

PloS one 20110520 5

<h4>Background</h4>Transforming growth factor beta 1 (TGFβ1) plays a major role in many lung diseases including lung cancer, pulmonary hypertension, and pulmonary fibrosis. TGFβ1 activates a signal transduction cascade that results in the transcriptional regulation of genes in the nucleus, primarily through the DNA-binding transcription factor SMAD3. The objective of this study is to identify genome-wide scale map of SMAD3 binding targets and the molecular pathways and networks affected by the T ...[more]

PMID: 21625455

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