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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis ARF6 and ARF8 mutants during flower development

ABSTRACT: The aim of this study was to examine the contribution of ARF6 and ARF8 to flower gene expression. Flowers from arf6 arf8 plants undergo a developmental arrest at approximately stage 12, just prior to flower opening. Flowers from wild-type, arf6/arf6 ARF8/arf8, and arf6 arf8 plants were separated into stage 1-10 flowers, stage 11+12 flowers, and stage 13-14 flowers to define the developmental stages at which ARF6 and ARF8 are required for gene expression.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Christine Ellis

PROVIDER: E-GEOD-2848 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation.

Nagpal Punita P Ellis Christine M CM Weber Hans H Ploense Sara E SE Barkawi Lana S LS Guilfoyle Thomas J TJ Hagen Gretchen G Alonso José M JM Cohen Jerry D JD Farmer Edward E EE Ecker Joseph R JR Reed Jason W JW

Development (Cambridge, England) 20050817 18

Pollination in flowering plants requires that anthers release pollen when the gynoecium is competent to support fertilization. We show that in Arabidopsis thaliana, two paralogous auxin response transcription factors, ARF6 and ARF8, regulate both stamen and gynoecium maturation. arf6 arf8 double-null mutant flowers arrested as infertile closed buds with short petals, short stamen filaments, undehisced anthers that did not release pollen and immature gynoecia. Numerous developmentally regulated g ...[more]

PMID: 16107481

Similar Datasets

Auxin Response Factor mediated flower gene expression

Project description:The aim of this study was to examine the contribution of ARF6 and ARF8 to flower gene expression. Flowers from arf6 arf8 plants undergo a developmental arrest at approximately stage 12, just prior to flower opening. Flowers from wild-type, ARF6/arf6 arf8/arf8, and arf6 arf8 plants were separated into stage 1-10 flowers, stage 11+12 flowers, and stage 13-14 flowers to define the developmental stages at which ARF6 and ARF8 are required for gene expression. Keywords: comparison of wild type and arf6 arf8 mutants

2005-06-30 | GSE2848 | GEO

Auxin induction in wild type and arf6 arf8 flowers

Project description:The aim of this study was to examine the roles of Auxin Response Factors (ARFs) in flower gene expression. Flowers from arf6 arf8 plants undergo a developmental arrest at approximately stage 12, just prior to flower opening. Wild-type, ARF6/arf6 arf8/arf8, and arf6 arf8 plants were treated with 10 uM indole-3-acetic acid for thirty minutes to identify genes that respond rapidly to auxin in an ARF6/ARF8-dependent manner. Keywords: auxin response; comparison of wild type and arf6 arf8 mutants

2005-06-30 | GSE2847 | GEO

Transcription profiling of Arabidopsis ARF6 and ARF8 mutants treated with indoleacetic acid (IAA)

Project description:The aim of this study was to examine the roles of Auxin Response Factors (ARFs) in flower gene expression. Flowers from arf6 arf8 plants undergo a developmental arrest at approximately stage 12, just prior to flower opening. Wild-type, ARF6/arf6 arf8/arf8, and arf6 arf8 plants were treated with 10 uM indole-3-acetic acid for thirty minutes to identify genes that respond rapidly to auxin in an ARF6/ARF8-dependent manner. Experiment Overall Design: Wild-type, ARF6/arf6 arf8/arf8, and arf6 arf8 plants were sprayed with 10 mM indole-3-acetic acid in 1% methanol, 0.05% Tween-20. Thirty minutes after treatment, flowers (stage 1-14) were collected, frozen in liquid nitrogen, and used for RNA extraction.

2007-07-11 | E-GEOD-2847 | biostudies-arrayexpress

Gene expression during Arabidopsis flower maturation

Project description:Flower maturation consists of several events that contribute to reproductive success as flowers open, including petal expansion, stamen filament elongation, pollen release, nectary maturation, stigma growth, and gynoecium maturation to support pollen tube growth. The Arabidopsis transcription factors ARF6 (Auxin Response Factor 6) and ARF8 regulate all of these processes, in part by activating jasmonate biosynthesis. Jasmonates in turn activate genes encoding the transcription factors MYB21 and MYB24, which mediate a subset of the processes controlled by ARF6 and ARF8. This experiment was designed to characterize gene expression in flowers before and after they open, and to determine how arf6 arf8 and myb21 myb24 mutation combinations affect these gene expression patterns. Three biological replicates were prepared at each of two developmental stages, stage 12 (oldest closed buds) and stage 13 (youngest open flowers), for three genotypes (Wild type, arf6-2 arf8-3, and myb21-5 myb24-5). For the mutant genotypes, stage 13 flowers do not actually open, so corresponding flowers of equivalent age were chosen based on the position of open flowers in wild-type inflorescences.

2011-12-10 | E-GEOD-32193 | biostudies-arrayexpress

Gene expression during Arabidopsis flower maturation

2011-12-10 | GSE32193 | GEO

Transcriptional profiling of Arabidopsis flower development of plants overexpressing ABAP1 gene

Project description:Gene expression profile of flower buds at stage 13, open flowers at stage 14 and siliques at stages 15/16, according to Smyth et al., 1990) of ABAP1 overexpressing (ABAP1OE) plants compared to wild type flower buds, open flowers and siliques (Col-0) using a a whole-genome oligonucleotide array (Operon) (platform accession number accession number GPL1077). ABAP1 is a negative regulator of the cell cycle that binds to transcription factors and represses their target gene expression, including pre-replication complex genes. All experiments were performed in triplicate.

2021-01-09 | GSE164480 | GEO

Reversible flower opening regulated by CPK16-dependent phosphorylation and activation of aquaporin PIP2;2 in Gentiana

Project description:Flower opening is important for successful pollination in many plant species, and some species repeat reversible flower opening and closing movements. This is thought to be due to the turgor pressure change caused by the water influx/efflux, which depends on osmotic oscillation in the cells. In some ornamental plants, it has been suggested that water channel aquaporin may play an important role in flower opening. However, the molecular mechanism(s) involved in flower movement are largely unknown. Using Gentiana flowers, which show reversible movement in response to temperature and light stimuli, as a model, we showed that reversible flower opening is regulated by aquaporin GsPIP2;2 and GsPIP2;7. In particular, phosphorylation of the C-terminal serine residue of GsPIP2;2 is important for the transport activity and correlates closely with the flower re-opening rate. Furthermore, GsPIP2;2 is phosphorylated and activated by GsCPK16, which is activated by elevated cytosolic Ca2+ levels in response to temperature and light stimuli. We propose that reversible flower opening is regulated by GsCPK16-dependent GsPIP2;2 phosphorylation and activation, with stimulus-induced calcium signals acting as triggers. CPK-dependent phosphorylation and activation of PIP2s may be one of the universal regulatory mechanisms for flower opening in plants.

2022-10-17 | PXD029169 | JPOST Repository

affy_petaldvt_lyon_rose: Flower/Petal initiation and development in roses

Project description:affy_petaldvt_lyon_rose. The objective is to identify genes involved in petal development in rose. We aim at identifying genes whose expression correlates with flower opening and scent emission. In this study, we used a microarray approach to compare the transcriptome of a scented rose flower (PF) versus non-scented rose flower (RF). Samples (petal tissues) were collected at the same time early in the afternoon. Total RNA was extracted using the Plant RNA kit (Macherey Nagel), and then used to hybridize Rosa-Affymetrix microarrays. Keywords: scented vs non-scented flowers 4 arrays - rose. Scented and non-scented flowers, 2 replicates each.

2011-08-31 | E-GEOD-18357 | biostudies-arrayexpress

Variations of Metabolites and Proteome from Lonicera japonica Thunb. Buds Blossom to Flowers under UV Radiation

Project description:Lonicera japonica Thunb., known as Jin Yin Hua or Japanese honeysuckle, is an herbal medicine in Asian countries. Its flowers have been used as folk medicine for clinical practice or used as food or making healthy beverage for 1500 years in China. To investigate the molecular developmental processes from L. japonica buds to flowers under UV radiation, comparative proteomics analyses of buds and flowers were performed. Fifty-four differential proteins were identified including 42 increased proteins and 12 decreased proteins. The abundance of proteins related to glycolysis, TCA/organic acid transformation, major carbohydrate metabolism, oxidative pentose phosphate, stress, secondary metabolism, hormone, and mitochondrial electron transport were increased during flower opening process under UV radiation. Six metabolites were identified and relatively quantified by LC-MS/MS in L. japonica buds and flowers. The 1,1-diphenyl-2-picrylhydrazyl assay revealed that antioxidant activity of L. japonica buds was better than that of flowers. These results suggest that UV-B radiation could induce the production of endogenous ethylene in L. japonica buds, which facilitate the buds blossom and activate the antioxidant system. Additionally, the higher content of metabolites and antioxidant capability in L. japonica buds indicates that L. japonica buds stage might be the better harvest time compared to the flower.

2017-01-18 | PXD005324 | Pride

Integration of developmental and nutritional cues determines timing of female flower opening in cucumber

Project description:The timing of flower opening is essential for pollination and thus seed production. Despite flower opening has been a long lasting topic for plant biologists, the underling molecular mechansim remains elusive. Here we used a unique cucumber line ‘6457’ that spontaneously produces super ovary with delayed corolla opening as material, and explored the physiological, cytological, nutritional and transcriptomic reasons for the delayed corolla opening in super ovary. Our data showed that cell division and cell expansion persisited for a longer period of time in the super ovary, especially during the green yellow bud stage and yellow bud stage. Similarly, RNA-seq analyses showed that RNA and protein synthesis related genes were upregulated during these two stages, which may account for the decreased nitrogen and phosphrate content in the super ovary. Further, activation of transcription factors were delayed in the green yellow bud stage, while signalling kinases related genes were upregulated in the yellow bud stage in the super ovary. Photosynthesis related genes were also significantly activated in the super ovary, which corresponds to the increased soluble suble content. Phytohoromones such as cytokinins and gibberllins were elevated in the super ovary. Consistently, cytokinins and gibberllins related genes showed significantly enhanced expression. Therefore, both developmental and nutritional factors regulate the timing of female flower opening in cucumber, and provide a valuable foundation for dissecting the underling regulatory pathways of flower opening in planta.

2016-07-25 | GSE76358 | GEO

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