CDNA microarray analysis of miR-1 transfected HeLa and NPC-TW01 cells
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Chin-Tarng Lin
PROVIDER: E-GEOD-28522 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
MicroRNA-1 induces apoptosis by targeting prothymosin alpha in nasopharyngeal carcinoma cells.
Wu Cheng-Der CD Kuo Yuan-Sung YS Wu Han-Chung HC Lin Chin-Tarng CT
Journal of biomedical science 20111107
<h4>Background</h4>MiR-1 (microRNA-1) has been used as a positive control in some microRNA experiments. We found that miR-1 transfection of nasopharyngeal carcinoma cells reveals a typical apoptotic process as shown by time-lapse microscopy so we investigated the mechanisms of miR-1 inducing apoptosis.<h4>Methods</h4>To confirm that miR-1 induces apoptosis, we used Annexin V and TUNEL staining and caspase assay. To determine that miR-1 directly targets genes that involve in apoptosis, we analyze ...[more]
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