Molecular characterization of immortalization of human MSCs by a combination of p53 knockdown and TERT overexpression

ABSTRACT: Gene expression profile of p53 knockdown MSCs or p53 knockdown+TERT MSCs was compared with that of control MSCs. Our data show p53 knockdown prolongs the lifespan of MSCs, and a combination of p53 knockdown and TERT overexpression is sufficient to immortalize MSCs. The results provide important information about the molecular basis underlying p53 knockdown in MSCs and immortalization-related genes of MSCs. Total RNA obtained from p53 knockdown MSCs or p53 knockdown+TERT MSCs from three patients were compared with control MSCs.

ORGANISM(S): Homo sapiens

SUBMITTER: Tongming Liu 

PROVIDER: E-GEOD-28546 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress