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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression profiles of human primary keratinocytes differentiated with TPA

ABSTRACT: Human primary keratinocytes were left untreated or treated for 48 hr with 12-O-tetradecanoylphorbol-13-acetate (TPA) and expression profiles were compared to find long ncRNAs expressed and differentially regulated upon differentiation. 4 replicates of minus TPA and 3 of plus TPA with dye-swap. Custom-made microarray from Agilent.

ORGANISM(S): Homo sapiens

SUBMITTER: Ulf Ørom

PROVIDER: E-GEOD-28552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Long noncoding RNAs with enhancer-like function in human cells.

Ørom Ulf Andersson UA Derrien Thomas T Beringer Malte M Gumireddy Kiranmai K Gardini Alessandro A Bussotti Giovanni G Lai Fan F Zytnicki Matthias M Notredame Cedric C Huang Qihong Q Guigo Roderic R Shiekhattar Ramin R

Cell 20101001 1

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in hum ...[more]

PMID: 20887892

Similar Datasets

Expression profiles of human primary keratinocytes differentiated with TPA

Project description:Human primary keratinocytes were left untreated or treated for 48 hr with 12-O-tetradecanoylphorbol-13-acetate (TPA) and expression profiles were compared to find long ncRNAs expressed and differentially regulated upon differentiation.

2011-04-12 | GSE28552 | GEO

Effect of RGRN-305 on TPA-induced skin inflammation in mice 4 hours post-TPA challenge

Project description:The aim was to demonstrate that pretreatment with RGRN-305 suppresses 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced alterations in gene expression.

2023-01-31 | GSE221997 | GEO

Enhancer-like function for long non-coding RNAs in human

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-04-12 | E-GEOD-23549 | biostudies-arrayexpress

CDDO drives transcriptomic and epigenetic reprogramming in response to TPA-induced JB6 cell neoplastic transformation [RNA-seq]

Project description:12-O-tetradecanoylphorbol-13-acetate (TPA) promotes skin carcinogenesis. CDDO is a potential antioxidative and antiinflammatory agent to prevent the TPA-induced skin cell transformation at nanomolar scale. We characterized the transcriptome, CpG methylome, and pathway network of JB6 cells treated with TPA and TPA + CDDO using RNA sequencing, methyl sequencing, and QIAGEN Ingenuity Pathway Analysis.

2022-06-18 | GSE206167 | GEO

CDDO drives transcriptomic and epigenetic reprogramming in response to TPA-induced JB6 cell neoplastic transformation [Methyl-seq]

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Single-cell RNA (10X Chromium) of mouse DMBA/TPA treated or control back skin and matched livers (2) (IKMB) 2022

Project description:We generated single cell transcriptomes from full thickness skin biopsies in mouse to quantify the skin cell types found in this species (control samples). To study how mouse skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 10 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

2023-11-21 | E-MTAB-12456 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of mouse DMBA/TPA treated or control back skin and matched livers (1) (DKFZ) 2022

2023-11-21 | E-MTAB-12454 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK B) 2022

Project description:We generated single cell transcriptomes from full thickness skin biopsies in naked mole-rat to quantify the skin cell types found in this species (control samples). To study if and how naked mole-rat skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment traditionally performed in mice, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 12 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

2023-11-21 | E-MTAB-12461 | biostudies-arrayexpress

Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK A) 2022

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Transcription profiling of human erythroleukemia K562 cells during TPA-induced megakaryocyte differentiation

Project description:Analysis of differential gene expression during TPA-induced megakaryocyte differentiation of human erythroleukemia K562 cells. The transcription profile of untreated vs TPA-treated K562 cells for 2 days was compared using gene microarrays.

2009-09-01 | E-MEXP-2213 | biostudies-arrayexpress

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