Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression of Stem Cell Pluripotency genes in mASCs and ES, iPS, and mi-iPS cells

ABSTRACT: Comparison of TaqMan Gene Signature Arrays (Mouse Stem Cell Pluripotency Array) was represented in mASCs and ES, iPS, and mi-iPS cells. Comparison of TaqMan Gene Signature Arrays (Mouse Stem Cell Pluripotency Array) was represented in mASCs and ES, iPS, and mi-iPS cells.

ORGANISM(S): Mus musculus

SUBMITTER: hideshi ishii

PROVIDER: E-GEOD-28586 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Reprogramming of mouse and human cells to pluripotency using mature microRNAs.

Miyoshi Norikatsu N Ishii Hideshi H Nagano Hiroaki H Haraguchi Naotsugu N Dewi Dyah Laksmi DL Kano Yoshihiro Y Nishikawa Shinpei S Tanemura Masahiro M Mimori Koshi K Tanaka Fumiaki F Saito Toshiyuki T Nishimura Junichi J Takemasa Ichiro I Mizushima Tsunekazu T Ikeda Masataka M Yamamoto Hirofumi H Sekimoto Mitsugu M Doki Yuichiro Y Mori Masaki M

Cell stem cell 20110601 6

Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature ...[more]

PMID: 21620789

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Project description:In this study we have compared functional and molecular properties of highly purified murine induced pluripotent stem (iPS) cell- and embryonic stem (ES) cell-derived cardiomyocytes (CM). In order to obtain large amounts of purified CM, we have generated a transgenic murine iPS cell line, which expresses puromycin resistance protein N-acetyltransferase and EGFP under the control of the cardiomyocyte-specific α-myosin heavy chain promoter (alphaMHC-Puro-IRES-GFP, aPiG). We demonstrate that murine aPIG-iPS and aPIG-ES cells differentiate into spontaneously beating CM at comparable efficiencies. When selected with puromycin both cell types yielded more than 97% pure population of CMs. Both aPIG-iPS and aPIG-ES cell-derived CM express typical cardiac transcripts and structural proteins and possess similar sarcomeric organization. Action potential recordings revealed that iPS- and ES cell-derived CM respond to beta-adrenergic and muscarinic receptor modulation, express functional voltage-gated sodium, calcium and potassium channels and possess comparable current densities. Comparison of global gene expression profiles of CM generated from iPS and ES cells revealed that both cell types cluster close to each other but are highly distant to undifferentiated ES or iPS cells as well as unpurified iPS and ES cell-derived embryoid bodies (EB). Both iPS and ES cell-derived CMs express genes and functional categories typical for CM. They are enriched in genes involved in transcription and genes coding for structural proteins involved in cardiac muscle contraction and relaxation. They also express genes involved in heart and muscle developmental processes, ion export and ion binding processes and various metabolic processes for ATP synthesis. These CMs downregulate genes involved in immune response, cell cycle and cell division, thus demonstrating the CMs population is mitotically inactive. Most surface signaling pathways are also downregulated. Thus, a transgenic aPiG-iPS cell line can provide a robust supply of highly purified and functional CMs for future in vitro and in vivo studies.

Dataset Information

Expression of Stem Cell Pluripotency genes in mASCs and ES, iPS, and mi-iPS cells

Publications

Reprogramming of mouse and human cells to pluripotency using mature microRNAs.

Similar Datasets

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