Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells

ABSTRACT: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies. In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. Using immunocytochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including astrocytomas and glioblastomas. To investigate the role of HOXD9 in gliomas, we silenced its expression in the glioma cell line U87 using HOXD9-specific siRNA, and observed decreased cell proliferation, cell cycle arrest, and induction of apoptosis. It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival. The HOXD9 gene was highly expressed in a side population (SP) of SK-MG-1 cells that was previously identified as an enriched-cell fraction of glioma cancer stem-like cells. HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. Finally, we cultured human glioma cancer stem cells (GCSCs) from patient specimens found with high expression of HOXD9 in GCSCs compared with normal astrocyte cells and neural stem/progenitor cells (NSPCs). Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. we analyzed the expression and function of HOXD9 in human gliomas and found high expression of HOXD9 in GCSCs. HOXD9 contributes to cell proliferation and/or survival in glioma cells and glioma cancer stem-like cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Masanao Tabuse

PROVIDER: E-GEOD-28618 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells.

Tabuse Masanao M Ohta Shigeki S Ohashi Yohei Y Fukaya Raita R Misawa Aya A Yoshida Kazunari K Kawase Takeshi T Saya Hideyuki H Thirant Cécile C Chneiweiss Hérve H Matsuzaki Yumi Y Okano Hideyuki H Kawakami Yutaka Y Toda Masahiro M

Molecular cancer 20110522

<h4>Background</h4>HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. They also behave as oncogenes in some malignancies.<h4>Results</h4>In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. Using immunohistochemistry, we observed HOXD9 protein expression in human brain tumor tissues, including ast ...[more]

PMID: 21600039

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Dataset Information

Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells

Publications

Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells.

Similar Datasets

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