Transcription profiling of mouse skeletal muscle to detect genes that regulate motor axon growth and differentiation

ABSTRACT: These experiments are designed to discover genes that are expressed selectively by synaptic nuclei in skeletal muscle with the particular goal of identifying genes that regulate motor axon growth and differentiation. We plan to isolate RNA from the dissected synaptic region of skeletal muscle and from the non-synaptic region of skeletal muscle and to identify the genes that are expressed at higher levels in the synaptic than non-synaptic region. Previously, we showed that motor axons fail to stop and differentiate in mice lacking MuSK, a receptor tyrosine kinase that is activated by motor neuron-derived Agrin. We hypothesize that MuSK activation normally leads to the production of a retrograde stop/differentiation signal that is encoded by a gene that is expressed preferentially in synapt

ORGANISM(S): Mus musculus

SUBMITTER: Elizabeth Salomon 

PROVIDER: E-GEOD-2873 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress