Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Small RNA sequencing in nine-day old nutrient-replete and N or P-limited wild type Arabidopsis seedlings


ABSTRACT: Identification of nutrient-specific microRNA expressed in Arabidopsis seedlings Small RNA sequencing of Arabidopsis wild type ecotype Columbia (Col-0) seedling under different nutrient limitation conditions

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Patrick May 

PROVIDER: E-GEOD-28733 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of nutrient-responsive Arabidopsis and rapeseed microRNAs by comprehensive real-time polymerase chain reaction profiling and small RNA sequencing.

Pant Bikram Datt BD   Musialak-Lange Magdalena M   Nuc Przemyslaw P   May Patrick P   Buhtz Anja A   Kehr Julia J   Walther Dirk D   Scheible Wolf-Rüdiger WR  

Plant physiology 20090522 3


Comprehensive expression profiles of Arabidopsis (Arabidopsis thaliana) MIRNA genes and mature microRNAs (miRs) are currently not available. We established a quantitative real-time polymerase chain reaction platform that allows rapid and sensitive quantification of 177 Arabidopsis primary miR transcripts (pri-miRs). The platform was used to detect phosphorus (P) or nitrogen (N) status-responsive pri-miR species. Several pri-miR169 species as well as pri-miR398a were found to be repressed during  ...[more]

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