Isolation and characterization of stem-like cells from a human ovarian cancer cell line

ABSTRACT: Increasing evidence supports the existence of a subpopulation of cancer cells capable of self-renewal and differentiation into diverse cell lineages. These cancer stem-like or cancer initiating cells (CICs) also demonstrate resistance to chemo- and radiotherapy and may function as a primary source of cancer recurrence. We report here on the isolation and in vitro propagation of multicellular ovarian cancer spheroids from a well-established ovarian cancer cell line (OVCAR-3). Cells forming these spheroids display self-renewal potential, the ability to produce differentiated progeny and increased expression of genes previously associated with CICs. The spheroid-derived cells (SDCs) also demonstrate higher invasiveness, migration potential and enhanced resistance to standard anticancer agents

ORGANISM(S): Homo sapiens

SUBMITTER: Roman Mezencev 

PROVIDER: E-GEOD-28799 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress