Chromosome-biased binding and gene reguation by the C. elegans DRM complex [ChIP-chip]

ABSTRACT: DRM is a conserved transcription factor complex that includes E2F/DP and pRB family proteins and plays important roles in development and cancer. Here we analyze genome-wide binding and function of the C. elegans DRM subunit LIN-54. We demonstrate that LIN-54 DNA-binding activity is required for the DRM complex to efficiently bind and regulate target genes containing adjacent putative E2F/DP and LIN-54 binding sites. We show that LIN-54 binds to the promoters of genes involved in cell division, development, and reproduction, and acts differently in the germline versus the soma. The E2F/DP-LIN-54 binding motif, individual target genes, and overall DRM function are conserved among worms, flies, and humans. Despite this conservation, we discovered one striking feature of C. elegans DR

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Kirsten Hagstrom 

PROVIDER: E-GEOD-28852 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress