Changes in the transcriptome of Entamoeba histolytica accompany the epigenetic silencing of the amoebapore gene.

ABSTRACT: We previously showed that epigenetic transcriptional silencing of the amoebapore gene (Ehap-a) in E. histolytica G3 trophozoites was accompanied by the down-regulation of two other members of the saposin-like gene family (Ehap-b, and EhSaplip1). Comparison of the transcriptomes of G3 trophozoites with those of the parent HM-1:IMSS strain revealed 72 additional transcripts that were either down- or up-regulated at least 7 fold. One of the polyA+, up-regulated (>200) transcripts (459.m00030), had the potential to encode a 66 amino acid protein. Antibodies raised against a synthetic N-terminal peptide of the gene did not recognize any such protein in trophozoite lysates. Trophozoite cultures grown with the proteasome inhibitor MG-132 to prevent protein degradation, also did not have any det

ORGANISM(S): Entamoeba histolytica

SUBMITTER: Carol Gilchrist 

PROVIDER: E-GEOD-28883 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress