Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Replication initiation sites in cancer cells

ABSTRACT: Reads from massively parallel sequencing of RNA primed, short nascent strands from asynchronously growing cancer cells (K562, MCF7). Newly replicated DNA was isolated based on size (400-800 bp) and the presence of a short RNA stretch at the 5' end using lambda exonuclease. Purified nascent strands were analyzed using massively parallel sequencing. Sheared genomic DNA was sequenced as a control.

ORGANISM(S): Homo sapiens

SUBMITTER: Mirit Aladjem 

PROVIDER: E-GEOD-28911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

This report investigates the mechanisms by which mammalian cells coordinate DNA replication with transcription and chromatin assembly. In yeast, DNA replication initiates within nucleosome-free regions, but studies in mammalian cells have not revealed a similar relationship. Here, we have used genome-wide massively parallel sequencing to map replication initiation events, thereby creating a database of all replication initiation sites within nonrepetitive DNA in two human cell lines. Mining this  ...[more]

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