MiR-135b exerts metastatic activity in the ErbB2 driven mammary carcinogenesis

ABSTRACT: In view of the roles played by miRNAs in cancer progression, we performed a microarray analysis aimed at identifying miR-135b target genes in breast cancer cells. Detection of transcripts affected by silencing of mir-135b was done using a time course at 24, 48 and 72 h both transfecting cell with mir-135b specific miRIDIAN microRNA Hairpin Inhibitor or miRIDIAN microRNA Hairpin Inhibitor negative control. Each experimental condition was repeated three times and analysed on on MOUSEREF-8 V2 Illumina bead chips. Genome studio was used to generate raw intensity signal without normalization. Data log2 transformation and loess normalization was performed with oneChannelGUI. Data were filtered using an IQR filter (i.e. only genes characterized by an IQR > 0.25, were retained for further analys

ORGANISM(S): Mus musculus

SUBMITTER: Raffaele Calogero 

PROVIDER: E-GEOD-28949 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress