Principles Governing Dicer-2 Substrate Selection are Revealed by Characterizing the RNAi Response to Virus Infection and Exogenous dsRNA

ABSTRACT: Background: RNA silencing pathways play critical roles in gene regulation, virus infection, and transposon control. RNA interference (RNAi) is mediated by small interfering RNAs (siRNAs), which are liberated from double stranded (ds) RNA precursors by Dicer and direct the RNA-induced silencing complex (RISC) to target transcripts. Recent efforts have uncovered important principles governing small RNA (smRNA) sorting into RISC, yet mechanisms defining substrate selection by Dicer proteins remain uncharacterized. Methodology: To better characterize Dicer-2 substrates in Drosophila, we examined the antiviral RNAi response, which generates virus-derived siRNAs from viral RNA. Using high-throughput sequencing, we found that diverse viruses were uniquely targeted; substrates included dsRNA repli

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Qi Zheng 

PROVIDER: E-GEOD-28957 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress