High-throughput sequencing of small RNAs in Marsilea quadrifolia

ABSTRACT: Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Marsilea quadrifolia tissues (leaves, roots and drought-treated leaves). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features as size distribution, tissue-specific regulation and sequence conservation between diff

ORGANISM(S): Marsilea quadrifolia

SUBMITTER: Emanuele De Paoli 

PROVIDER: E-GEOD-28970 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress