Pronounced and Extensive Microtubule Defects in a Saccharomyces cerevisiae DIS3 Mutant

ABSTRACT: The recently proposed exozyme hypothesis posits that subunits of the RNA processing exosome assemble into structurally distinct protein complexes that function in disparate cellular compartments and RNA metabolic pathways. Here, in a genetic test of this hypothesis, we examine the role of Dis3 -- an essential polypeptide with endo- and 3' to 5' exo-ribonuclease activity -- in cell cycle progression. We present several lines of evidence that perturbation of DIS3 affects microtubule (MT) localization and structure in Saccharomyces cerevisiae. Cells with a DIS3 mutation: (i) accumulate anaphase and pre-anaphase mitotic spindles; (ii) exhibit spindles that are mis-oriented and displaced from the bud neck; (iii) harbor elongated spindle-associated astral MTs; (iv) have an increased G1 astral

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Daniel Kiss 

PROVIDER: E-GEOD-29018 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress