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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

ABSTRACT: We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain. The ade3 mutant strain (Y4143) was compared to the WT strain (Y2946). For each comparison, we made 2 technical replicates, swapping dyes.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: sophie lemoine

PROVIDER: E-GEOD-29021 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Physiological and toxic effects of purine intermediate 5-amino-4-imidazolecarboxamide ribonucleotide (AICAR) in yeast.

Hürlimann Hans C HC Laloo Benoît B Simon-Kayser Barbara B Saint-Marc Christelle C Coulpier Fanny F Lemoine Sophie S Daignan-Fornier Bertrand B Pinson Benoît B

The Journal of biological chemistry 20110712 35

5-Amino-4-imidazolecarboxamide ribonucleotide 5'-phosphate (AICAR) is a monophosphate metabolic intermediate of the de novo purine synthesis pathway that has highly promising metabolic and antiproliferative properties. Yeast mutants unable to metabolize AICAR are auxotroph for histidine. A screening for suppressors of this phenotype identified recessive and dominant mutants that result in lowering the intracellular AICAR concentration. The recessive mutants affect the adenosine kinase, which is ...[more]

PMID: 21757731

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