Transcriptional Profiles Induced by the Aryl Hydrocarbon Receptor Agonists 2,3,7,8-Tetrachlorodibenzo-p-dioxin, 2,3,7,8-Tetrachlorodibenzofuran and 2,3,4,7,8-Pentachlorodibenzofuran in Primary Rat Hepatocytes
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ABSTRACT:
ORGANISM(S): Rattus norvegicus
SUBMITTER: Craig Rowlands
PROVIDER: E-GEOD-29095 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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Project description:A toxicogenomics approach was used to qualitatively and quantitatively compare gene expression changes in rat primary hepatocytes exposed to 2,3,4,7,8-pentachlorodibenzofuran (4-PeCDF) or 2,3,7,8-tetrachlorodibenzofuran (TCDF). Hepatocytes from five individual rats were exposed for 24 hours to 11 concentrations of each chemical ranging from 0.00001 nM to 100 nM and a vehicle control. 115 microarrays representing primary hepatocytes from 5 rats, each exposed to 11 doses of each chemical (PeCDF and TCDF) and vehicle controls. Arrays were completed with a one-color design.
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