RNA-Seq anlalysis of prostate cancer cell lines using Next Generation Sequencing

ABSTRACT: Beginning with precursor lesions, aberrant DNA methylation marks the entire spectrum of prostate cancer progression. We mapped the global DNA methylation patterns in selected prostate tissues and cell lines using Methylplex-Next Generation Sequencing (M-NGS). Hidden Markov Model based next generation sequence analysis identified ~68,000 methylated regions per sample. While global CpG Island (CGI) methylation was not differential between benign adjacent and cancer samples, overall promoter CGI methylation increased from ~12.6% in benign samples to 19.3% and 21.8% in localized and metastatic cancer tissues, respectively. We found distinct patterns of promoter methylation around transcription start sites, where methylation occurred not only on the CGIs, but also on flanking regions and CGI sp

ORGANISM(S): Homo sapiens

SUBMITTER: Jung Kim 

PROVIDER: E-GEOD-29155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress