Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic cell lines co-occupied with lineage specific regulators (GATA1, GATA2, CEBPA)

ABSTRACT: Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration. This represents the human ChIP-seq portion of this dataset on leukemia cell lines. Human hematopoietic cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Tcf7l2 (Santa Cruz SC-8631),Gata1 (Santa Cruz SC-265), Gata2 (Santa Cruz SC-9008), CEBPA (SC-9314) or H3K4me1 (Abcam ab8895).

ORGANISM(S): Homo sapiens

SUBMITTER: Teresa Bowman 

PROVIDER: E-GEOD-29195 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

BMP and Wnt signaling pathways control essential cellular responses through activation of the transcription factors SMAD (BMP) and TCF (Wnt). Here, we show that regeneration of hematopoietic lineages following acute injury depends on the activation of each of these signaling pathways to induce expression of key blood genes. Both SMAD1 and TCF7L2 co-occupy sites with master regulators adjacent to hematopoietic genes. In addition, both SMAD1 and TCF7L2 follow the binding of the predominant lineage  ...[more]

Similar Datasets

Genomics Genome-wide location analysis of WNT (Tcf7l2) and BMP (SMAD1) in human hematopoeitic progenitors co-occupied with lineage specific regulators (GATA1, GATA2)
2011-10-07 | E-GEOD-29194 | biostudies-arrayexpress
Genomics Genome-wide location analysis of BMP (SMAD1) in mouse erythroid progenitors co-occupted with lineage specific regulators (GATA1, GATA2)
2011-10-07 | E-GEOD-29193 | biostudies-arrayexpress
Genomics Core transcriptional regulatory circuit controlled by the tal1 complex in human t-cell acute lymphoblastic leukemia (Subseries)
2012-03-14 | E-GEOD-33850 | biostudies-arrayexpress
Genomics ChIP-Seq of TAL1 and its regulatory partners in T-ALL cells
2012-02-09 | E-GEOD-29180 | biostudies-arrayexpress
Genomics TAL1 complex in human T-cell acute lymphoblastic leukemia (RPMI-8402)
2012-07-09 | E-GEOD-39179 | biostudies-arrayexpress
Genomics Transcription factor ChIP-seq in expanded human hematopoietic stem and progenitor cells
2011-01-26 | E-GEOD-26014 | biostudies-arrayexpress
Genomics P15 Rat Sciatic Nerve ChIP-Chip with NuRD complex components
2011-07-26 | GSE30890 | GEO
Genomics Total RNA Pol2 ChIP-seq in mES cells treated with flavopiridol (Cdk9 inhibitor) after DSIF and NELF knockdown
2011-01-19 | E-GEOD-26658 | biostudies-arrayexpress
Proteomics Identifying the N-terminus of SLX4Nter in HeLa KO30 cells
2022-02-16 | PXD029062 | Pride
Genomics ChIP-Seq of human MCF7 and HEPG2 cells using antibodies against CTCF, STAG1, RAD2, ERa and CEBPa
2010-05-13 | E-TABM-828 | biostudies-arrayexpress