Global Gene Expression Profiles of Visceral Adipose in Females with Type 2 Diabetes.

ABSTRACT: Gene expression profiles of biopsy samples of visceral adipose of three female patients of type 2 diabetes and three non-diabetic female patients were generated using Illumina HumanHT-12 v3 Expression BeadChip arrays. The primary indications of surgery were non-infective and non-malignant conditions, namely, cholelethiasis, hernia and trauma. Three biological replicates with four technical replicates each were used to generate expression profiles. Total cellular RNA from biopsy samples was isolated using mirVana™ miRNA Isolation Kit (Ambion, Austin, TX). The quantity and the quality of RNA samples were determined using Nanodrop-1000 (Thermo Fischer Scientific, Wilmington, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), in that order. RNA samples with a RIN (RNA I

ORGANISM(S): Homo sapiens

SUBMITTER: Abhay Sharma 

PROVIDER: E-GEOD-29231 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress