Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

An Integrated Analysis of miRNA and mRNA Expressions of Non-small Cell Lung Cancer

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE29248: Genome-wide analysis of microRNA expression in Non-small Cell Lung Cancer GSE29249: Genome-wide analysis of gene expression in Non-small Cell Lung Cancer Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: lina ma

PROVIDER: E-GEOD-29250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.

Ma Lina L Huang Yanyan Y Zhu Wangyu W Zhou Shiquan S Zhou Jihang J Zeng Fang F Liu Xiaoguang X Zhang Yongkui Y Yu Jun J

PloS one 20111027 10

Using DNA microarrays, we generated both mRNA and miRNA expression data from 6 non-small cell lung cancer (NSCLC) tissues and their matching normal control from adjacent tissues to identify potential miRNA markers for diagnostics. We demonstrated that hsa-miR-96 is significantly and consistently up-regulated in all 6 NSCLCs. We validated this result in an independent set of 35 paired tumors and their adjacent normal tissues, as well as their sera that are collected before surgical resection or c ...[more]

PMID: 22046296

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Project description:The tumor microenvironment strongly influences cancer development, progression and metastasis. The role of carcinoma-associated fibroblasts (CAFs) in these processes and their clinical impact has not been studied systematically in non-small cell lung carcinoma (NSCLC). We established primary cultures of CAFs and matched normal fibroblasts (NFs) from 15 resected NSCLC. We demonstrate that CAFs have greater ability than NFs to enhance the tumorigenicity of lung cancer cell lines. Microarray gene expression analysis of the 15 matched CAF and NF cell lines identified 46 differentially expressed genes, encoding for proteins that are significantly enriched for extracellular proteins regulated by the TGF-beta signaling pathway. We have identified a subset of 11 genes that formed a prognostic gene expression signature, which was validated in multiple independent NSCLC microarray datasets. Functional annotation using protein-protein interaction analyses of these and published cancer stroma-associated gene expression changes revealed prominent involvement of the focal adhesion and MAPK signalling pathways. Fourteen (30%) of the 46 genes also were differentially expressed in laser-capture micro-dissected corresponding primary tumor stroma compared to the matched normal lung. Six of these 14 genes could be induced by TGF-beta1 in NF. The results establish the prognostic impact of CAF-associated gene expression changes in NSCLC patients. This SuperSeries is composed of the following subset Series: GSE22862: Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer [expression profiling_CAFs] GSE22863: Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer [expression profiling_NSCLC stroma] GSE27284: Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer [methylation profiling] GSE27289: Prognostic Gene Expression Signature of Carcinoma Associated Fibroblasts in Non-Small Cell Lung Cancer [genome variation profiling]

Dataset Information

An Integrated Analysis of miRNA and mRNA Expressions of Non-small Cell Lung Cancer

Publications

An integrated analysis of miRNA and mRNA expressions in non-small cell lung cancers.

Similar Datasets

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