Expression data of pmr1M-bM-^HM-^F mutants

ABSTRACT: The yeast PMR1 (ATP2C1) gene codes for the eukaryotic prototype of a high affinity P-type ATPase required for Ca2+/Mn2+ transport into the Golgi. Cells lacking PMR1 exhibit multiple genetic interactions with genes involved in DNA recombination and replication, a fact that is not yet understood. We find that deletion of PMR1 causes a delay in DNA replication initiation, progression and G2/M transition and induces the transcriptional up-regulation of genes involved in cell cycle regulation, including CLB5 and SWE1. Interestingly, pmr1M-bM-^HM-^F clb5M-bM-^HM-^F double mutants exhibit a dramatic delay in DNA replication and increased DNA breakage, while endoreplication and the formation of multi-nucleated, giant yeast is observed in pmr1M-bM-^HM-^F swe1M-bM-^HM-^F cells. Because these phenoty

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Nestor Garcia-Rodriguez 

PROVIDER: E-GEOD-29420 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress