Integrated approaches reveal determinants of genome-wide binding and function of the transcription factor Pho4

ABSTRACT: Binding of transcription factors to DNA is a key regulatory step in the control of gene expression. DNA sequences with high affinity for transcription factors occur more frequently in the genome than instances of genes bound or regulated by these factors. Although several mechanisms have been identified that influence the specificity of transcriptional regulation, it is not known if these can explain the observed genome-wide pattern of binding or regulation for a given transcription factor. We used genome-wide approaches to study how trans influences shape the binding and regulatory landscape of Pho4, a budding yeast transcription factor that activates gene expression in response to phosphate limitation. We find that nucleosomes significantly restrict the sites to which Pho4 binds. At

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Xu Zhou 

PROVIDER: E-GEOD-29506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress