Paneth cell granule dissolution and bacterial colonization in the intestine of CF mice

ABSTRACT: Paneth cells of intestinal crypts contribute to host defense by producing antimicrobial peptides that are packaged as granules for secretion into the crypt lumen. Here, we provide evidence using light and electron microscopy that postsecretory Paneth cell granules undergo limited dissolution and accumulate within the intestinal crypts of cystic fibrosis (CF) mice. On the basis of this finding, we evaluated bacterial colonization and expression of two major constituents of Paneth cells, i.e., {alpha}-defensins (cryptdins) and lysozyme, in CF murine intestine. Paneth cell granules accumulated in intestinal crypt lumens in both untreated CF mice with impending intestinal obstruction and in CF mice treated with an osmotic laxative that prevented overt clinical symptoms and mucus accretion. Ult

ORGANISM(S): Mus musculus

SUBMITTER: Lara Gawenis 

PROVIDER: E-GEOD-2958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress