Circadian Clock Activity in Mouse and Human CD4+ T Cells

ABSTRACT: Though it is well established that immunological functions of CD4+ T cells are time of day-dependent, the underlying molecular mechanisms remain largely obscure. To address the question whether T cells themselves harbor a functional clock driving circadian rhythms of immune function, we analyzed clock gene expression and immune responses of CD4+ T cells purified from blood of healthy subjects at different time points throughout the day. Circadian clock function as well as immune function was further analyzed in cultivated T cells and circadian clock reporter systems. We found robust rhythms of clock gene expression as well as, after stimulation, of IFN-g production and CD40L expression in both freshly isolated and in cultured CD4+ T cells. Moreover, circadian luciferase reporter activities

ORGANISM(S): Homo sapiens

SUBMITTER: Lennart Opitz 

PROVIDER: E-GEOD-29583 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress