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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Identification of avian RIG-I responsive genes during influenza infection

ABSTRACT: Transcriptional profiling of chicken embryonic fibroblasts (DF-1 cells) comparing the effects of chicken cells transfected with duck RIG-I compared to empty-vector transfected cells following with low or highly pathogenic avian influenza. Goal was to determine the effects of duck RIG-I on influenza-induced immune gene expression. Two-condition experiment. Biological replicates for low pathogenic avian influenza infection: 3 empty-vector transfected replicates, 3 RIG-I transfected replicates. Biological replicates for highly pathogenic influenza infection: 2 empty-vector transfected replicates, 2 RIG-I transfected replicates.

ORGANISM(S): Gallus gallus

SUBMITTER: Yong-Dong Wang

PROVIDER: E-GEOD-29596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of avian RIG-I responsive genes during influenza infection.

Barber Megan R W MR Aldridge Jerry R JR Fleming-Canepa Ximena X Wang Yong-Dong YD Webster Robert G RG Magor Katharine E KE

Molecular immunology 20121204 1

Ducks can survive infection with highly pathogenic avian influenza viruses that are lethal to chickens. We showed that the influenza detector, RIG-I can initiate antiviral responses in ducks, but this gene is absent in chickens. We can reconstitute this pathway by transfecting chicken DF-1 embryonic fibroblast cells with duck RIG-I, which augments their antiviral response to influenza and decreases viral titer. However, the genes downstream of duck RIG-I that mediate this antiviral response to i ...[more]

PMID: 23220072

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Identification of avian RIG-I responsive genes during influenza infection

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Identification of avian RIG-I responsive genes during influenza infection

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Genome wide host gene expression analysis in chicken lungs infected with avian influenza viruses

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Expression data from low- and high-pathogenicity avian influenza-infected chicken and duck cells

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